Experiment

Organic Molecules I Pinal Patel Joseph LaMattina September 08, 2010 Introduction: This lab is plastered to carry knocked out(p) analyses to determine which innate molecules are evidence in a series of mysterious supplements. There are four staining tooth roots such as Ninhydrin, Lugols regent, Biuret reagent, and benedicks reagent provided to determine the armorial bearing of starch, sugar (monosaccharide), protein, and aminic stifling. The presence of each organic molecule is determined by smorgasbord the staining final results to particular organic molecules fade away agent and noticing the budge of emblazon. individually staining answers detect antithetic organic molecule presence. Ninhydrin is a chemical solution utilise to detect ammonia or primary and secondary amines. The Ninhydrin is use intermix with amino acid solution and note the people of colour change in the solution (3). benedicks reagent is utilize to test t he presence of trim back sugars which includes monosaccharide. This reagent contains blue copper (II) ions (Cu2+) which are reduced to copper (I) (Cu+). The Benedicts reagent is mix with sugar solution and notice the discolour change in the solution (4). Lugols reagent is a solution of element iodin and green iodide in water.

This reagent is used as an indicator test for the presence of starch in the solution (1). Biurets reagent is make of potassium hydroxide (KOH) and hydrated copper (II) sulfate, together with potassium sodium tartrate. This reagent is used to detect the protein in the solution. The Biurets solution is mix with the protein solution and notice! the change of color in the solution (2). By figuring out all of the color change of organic molecules, test for the unknown solutions for the presence of the organic molecules. Method: Took clean test furnishs. For minus control, added 5 mL of water to the test tube and because added 1 mL of Lugols reagent. Covered the test tube with parafilm and mix it. Recorded the color change....If you want to get a full essay, order it on our website: OrderCustomPaper.com

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